Recombinant Anti-ErbB2 / HER2 Antibody [ERBB2/8142R] (A314919)

Anti-ErbB2 / HER2 Antibody [ERBB2/8142R]

Recombinant rabbit monoclonal [ERBB2/8142R] antibody to ErbB2 / HER2.

Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Flow Cytometry, IF, IHC-P

SKBR-3 cells. Human breast carcinoma tissue (IHC).

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IHC: 1-2 µg/ml

Human

Recombinant fragment (around Amino Acids 1055-1255) of human ERBB2 encoding cytoplasmic domain. The exact sequence is proprietary.

Rabbit

Monoclonal

ERBB2/8142R

kappa

Protein A/G chromatography.

200 µg/ml

185 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.