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Anti-ErbB2/HER2 (phospho Tyr1221 + 1222) Antibody (A11006)

Western Blot - Anti-ErbB2/HER2 (phospho Tyr1221 + 1222) Antibody (A11006) - Antibodies.com
$485
Product Datasheet

Rabbit polyclonal antibody to ErbB2/HER2 (phospho Tyr1221 + 1222) for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-ErbB2/HER2 (phospho Tyr1221 + 1222) Antibody
Description
Rabbit polyclonal antibody to ErbB2/HER2 (phospho Tyr1221 + 1222).
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human
Immunogen
A synthetic phosphorylated peptide around Y1221 & Y1222 of human HER2/ERBB2 (NP_004439.2).
Sequence
NLYYWD
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
185 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-ErbB2/HER2 (phospho Tyr1221 + 1222) Antibody (A11006) - Antibodies.comEnlarge Image

Western Blot - Anti-ErbB2/HER2 (phospho Tyr1221 + 1222) Antibody (A11006)

Western blot analysis of extracts of HeLa cells, using Anti-ErbB2/HER2 (phospho Tyr1221 + 1222) Antibody (A11006) at 1:1,000 dilution. HeLa cells were treated by EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
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