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Anti-ErbB3/HER3 Antibody (A12718)

Western Blot - Anti-ErbB3/HER3 Antibody (A12718) - Antibodies.com Western Blot - Anti-ErbB3/HER3 Antibody (A12718) - Antibodies.com
Knockout Validated
$485
Product Datasheet

Rabbit polyclonal antibody to ErbB3/HER3 for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-ErbB3/HER3 Antibody
Description
Rabbit polyclonal antibody to ErbB3/HER3.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1275-1342 of human ErbB3/HER3 (NP_001973.2).
Sequence
DYAAMGACPASEQGYEEMRAFQGPGHQAPHVHYARLKTLRSLEATDSAFDNPDYWHSRLFPKANAQRT
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
185 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-ErbB3/HER3 Antibody (A12718) - Antibodies.comEnlarge Image

Western Blot - Anti-ErbB3/HER3 Antibody (A12718)

Western blot analysis of extracts of various cell lines, using Anti-ErbB3/HER3 Antibody (A12718) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-ErbB3/HER3 Antibody (A12718) - Antibodies.comEnlarge Image

Western Blot - Anti-ErbB3/HER3 Antibody (A12718)

Western blot analysis of extracts from normal (control) and ErbB3/HER3 knockout (KO) 293T cells, using Anti-ErbB3/HER3 Antibody (A12718) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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