Anti-eRF1 Antibody (A15053)

Name: Anti-eRF1 Antibody
Brand: Antibodies.com
SKU: A15053
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to eRF1 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-eRF1 Antibody

Description

Rabbit polyclonal antibody to eRF1.

Applications

WB, ICC/IF

Dilutions

WB: 1:200-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 278-437 of human ETF1 (NP_004721.1).

Sequence

VKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDIMRYVLHCQGTEEEKILYLTPEQEKDKSHFTDKETGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMEYQGGDDEFFDLDDY

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ETF1, Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, Protein Cl1, RF1, SUP45L1, TB3-1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-eRF1 Antibody (A15053)

Western blot analysis of extracts of various cell lines, using Anti-eRF1 Antibody (A15053) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Immunofluorescence - Anti-eRF1 Antibody (A15053)

Immunofluorescence analysis of HeLa cells using Anti-eRF1 Antibody (A15053) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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