Anti-ERK1 (phospho Thr202 + Tyr204) + ERK2 (phospho Thr185 + Tyr187) Antibody [ARC1595] (A306747)

Western blot analysis of extracts of various cell lines, using Anti-ERK1 (phospho Thr202 + Tyr204) + ERK2 (phospho Thr185 + Tyr187) Antibody [ARC1595] (A306747) at 1:1,000 dilution. Both 293T cells and NIH/3T3 cells were treated by ATP(5 mM) at 30°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 10s.

Western blot analysis of extracts of C6 cells, using Anti-ERK1 (phospho Thr202 + Tyr204) + ERK2 (phospho Thr185 + Tyr187) Antibody [ARC1595] (A306747) at 1:1,000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-ERK1 (phospho Thr202 + Tyr204) + ERK2 (phospho Thr185 + Tyr187) Antibody [ARC1595] (A306747) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat fallopian tube using Anti-ERK1 (phospho Thr202 + Tyr204) + ERK2 (phospho Thr185 + Tyr187) Antibody [ARC1595] (A306747) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.