Anti-ERLIN1 Antibody (A89706)

Name: Anti-ERLIN1 Antibody
Brand: Antibodies.com
SKU: A89706
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to ERLIN1 for WB, IHC and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-ERLIN1 Antibody

Description

Rabbit polyclonal antibody to ERLIN1.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 259-348 of human ERLIN1 (NP_006450.2).

Sequence

KADAEYYAAHKYATSNKHKLTPEYLELKKYQAIASNSKIYFGSNIPNMFVDSSCALKYSDIRTGRESSLPSKEALEPSGENVIQNKESTG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

39 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

C10orf69, Endoplasmic reticulum lipid raft-associated protein 1, Erlin-1, KE04, KEO4, Protein KE04, SPFH domain-containing protein 1, SPFH1, Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-ERLIN1 Antibody (A89706)

Western blot analysis of extracts of various cell lines, using Anti-ERLIN1 Antibody (A89706) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-ERLIN1 Antibody (A89706)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-ERLIN1 Antibody (A89706) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Western Blot - Anti-ERLIN1 Antibody (A89706)

Immunoprecipitation analysis of 300µg extracts of DU145 cells using 3µg of Anti-ERLIN1 Antibody (A89706). This Western blot was performed on the immunoprecipitate using Anti-ERLIN1 Antibody (A89706) at a dilution of 1:1000.

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