Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Endoplasmic reticulum resident protein 18, Endoplasmic reticulum resident protein 19, ER protein 18, ER protein 19, hTLP19, Thioredoxin domain-containing protein 12, Thioredoxin-like protein p19, TLP19, TXNDC12
Western blot analysis of extracts of various cell lines, using Anti-ERp18 Antibody (A88647) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-ERp18 Antibody (A88647) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-ERp18 Antibody (A88647) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH-3T3 cells using Anti-ERp18 Antibody (A88647) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U-2 OS cells using Anti-ERp18 Antibody (A88647) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
![Western Blot - Anti-ERp18 Antibody [ARC2718] (A307306) - Antibodies.com](https://cdn.antibodies.com/image/catalog/307/A307306_1.jpg?profile=product_alternative)