Anti-Escherichia coli J5 LPS Antibody [GNE13-337.5 (2D7/1)] (A280523)

Anti-Escherichia coli J5 LPS Antibody [GNE13-337.5 (2D7/1)]

Mouse monoclonal [GNE13-337.5 (2D7/1)] antibody to Escherichia coli J5 LPS.

This antibody recognises E. coli J5 LPS. The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The J5 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).The clone 2D7/1 has also been found to react with K. pneumoniae, S. sonnei and S. typhimurium.

ELISA, WB

Bacterial

E. coli J5 cells.

Mouse

Monoclonal

GNE13-337.5 (2D7/1)

IgG2b

500 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Escherichia coli J5 LPS antibody, clone 2D7/1 recognizes E. coli J5 LPS. The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The J5 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).The clone 2D7/1 has also been found to react with K. pneumoniae, S. sonnei and S. typhimurium.