Rabbit polyclonal antibody to Estrogen Receptor-a (Ab-106)
Specificity
The antibody detects endogenous level of total Estrogen Receptor-a protein.
Applications
WB, IHC
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around aa.104~108 (S-P- S-P-L) derived from Human Estrogen Receptor-a.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from MCF7 cells using Estrogen Receptor-a (Ab-106) Antibody #21066 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a (Ab-106) Antibody #21066 (left) or the same antibody preincubated with blocking peptide (right).
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