Mouse monoclonal [ESR1/420] antibody to Estrogen Receptor alpha (CF®647).
Applications
WB, IP, IHC
Positive Control
MCF-7 cells or breast carcinoma tissue.
Dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/mL, WB: 1-2 µg/mL, IHC-P: 1-2 µg/mL. Directly conjugated primary antibodies may require increased working concentrations compared with indirect detection systems. Antibody concentration and assay conditions should be optimized for each application. A suggested starting concentration is 1-2 µg/mL for most assays.
Reactivity
Human
Immunogen
Recombinant human Estrogen Receptor alpha (ESR1) protein corresponding to amino acids 290-560.
Host
Mouse
Clonality
Monoclonal
Clone ID
ESR1/420
Isotype
IgG2a
Conjugate
CF®647
Excitation: 650nm, Emission: 665nm
Concentration
0.1 mg/mL
Predicted MW
67 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
Synonyms
ER, ER-alpha, ESR, ESR1, Estradiol receptor, Estrogen receptor, NR3A1, Nuclear receptor subfamily 3 group A member 1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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