Rabbit polyclonal antibody to Estrogen Receptor-α (Phospho-Ser102)
Specificity
The antibody detects endogenous levels of Estrogen Receptor-α only when phosphorylated at serine 102.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Serine102(L-N-S(p)-V-S) derived from Human Estrogen Receptor-α.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from HuvEc cells (Lane 2), using Estrogen Receptor-α (Phospho-Ser102) Antibody #11801. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Phospho-Ser102) antibody #11801 (left)or the same antibody preincubated with blocking peptide (right).
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