Rabbit polyclonal antibody to ETK (phospho Tyr566).
Specificity
This antibody detects endogenous levels of ETK only when phosphorylated at Tyr566.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:10000
Reactivity
Human, Mouse
Immunogen
Synthetic peptide derived from human ETK around the phosphorylation site of Tyr566 (amino acids 532-581).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
78kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Bone marrow tyrosine kinase gene in chromosome X protein, Cytoplasmic tyrosine-protein kinase BMX, Epithelial and endothelial tyrosine kinase, ETK, NTK38
Western Blot - Anti-ETK (phospho Tyr566) Antibody (A94301)
Western blot analysis of lysates from HeLa cells treated with Serum 20% 15' using Anti-ETK (phospho Tyr566) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human thyroid gland using Anti-ETK (phospho Tyr566) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
