Anti-ETV1 Antibody [ARC60301] (A329350)

Name: Anti-ETV1 Antibody [ARC60301]
Brand: Antibodies.com
SKU: A329350
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC60301] antibody to ETV1 for WB, IP, ChIP and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-ETV1 Antibody [ARC60301]

Description

Rabbit monoclonal [ARC60301] antibody to ETV1.

Applications

WB, IP, ChIP, ELISA

Positive Control

HeLa, SH-SY5Y, PC-3, SH-SY5Y, Mouse brain, Mouse kidney, Rat brain, Mouse brain.

Dilutions

WB: 1:500-1:1,000, IP: 0.5µg-4µg antibody for 400µg-800µg extracts of whole cells, ELISA: 1 µg/ml (starting concentration), ChIP: 5µg antibody for 10µg-15µg of Chromatin

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to amino acids 1-100 of human ETV1.

Sequence

MDGFYDQQVPYMVTNSQRGRNCNEKPTNVRKRKFINRDLAHDSEELFQDLSQLQETWLAEAQVPDNDEQFVPDYQAESLAFHGLPLKIKKEPHSPCSEIS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC60301

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

55 kDa

Predicted MW

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 0.05% BSA, 50% Glycerol, and 0.05% ProClin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ER81, ETS translocation variant 1, Ets-related protein 81

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-ETV1 Antibody [ARC60301] (A329350)

ETV1 expression in lysates from wild type (WT) and ETV1 knockdown (KD) HeLa cells analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-ETV1 Antibody [ARC60301] (A329350) at a dilution of 1:1000 and detected with chemiluminescence.

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Western Blot - Anti-ETV1 Antibody [ARC60301] (A329350)

ETV1 expression in SH-SY5Y cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-ETV1 Antibody [ARC60301] (A329350) at a dilution of 1:1000 and detected with chemiluminescence.

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Western Blot - Anti-ETV1 Antibody [ARC60301] (A329350)

ETV1 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-ETV1 Antibody [ARC60301] (A329350) at a dilution of 1:1000 and detected with chemiluminescence.

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Western Blot - Anti-ETV1 Antibody [ARC60301] (A329350)

ETV1 expression in 600 µg extracts of Mouse brain analyzed by western blot following immunoprecipitation using Anti-ETV1 Antibody [ARC60301] (A329350) at a dilution of 1:5000 and using 1 µg, respectively.