Anti-EZH2 Antibody (A14944)

Name: Anti-EZH2 Antibody
Brand: Antibodies.com
SKU: A14944

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This product is discontinued

Anti-EZH2 Antibody (A14944) has been discontinued and is no longer available.

View all EZH2 Antibodies.

Specifications

Name

Anti-EZH2 Antibody

Description

Rabbit polyclonal antibody to EZH2.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 133-252 of human EZH2/KMT6 (NP_001190176.1).

Sequence

YMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

98 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Enhancer of zeste homolog 2, ENX-1, Histone-lysine N-methyltransferase EZH2, KMT6, Lysine N-methyltransferase 6

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-EZH2 Antibody (A14944)

Western blot analysis of extracts of various cell lines, using Anti-EZH2 Antibody (A14944) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Immunohistochemistry - Anti-EZH2 Antibody (A14944)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-EZH2 Antibody (A14944) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-EZH2 Antibody (A14944)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-EZH2 Antibody (A14944) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-EZH2 Antibody (A14944)

Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-EZH2 Antibody (A14944) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.