This antibody targets the C-terminal region of human F2RL3, recognizing the non-phosphorylated isoform. It is optimized for Western blot analysis, supports immunoprecipitation from cell and tissue lysates, and is suitable for immunohistochemistry in cultured cells and tissue sections.
Applications
WB, IHC
Dilutions
WB: 1,000, IHC: 1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human F2RL3. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot validation of F2RL3 in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing F2RL3 were probed with anti-F2RL3 antibody (A334539) at 1:1000.
Immunohistochemical detection of F2RL3 in human small intestine. Small intestine sections were dewaxed, microwaved in citric acid, and incubated with anti-F2RL3 antibody (A334539) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.
Publishing research using Anti-F2RL3 Antibody (A334539)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-F2RL3 Antibody (A334539)
