Anti-Factor I / CFI Antibody [052.11.3 (3R/8)] (A278904)

Anti-Factor I / CFI Antibody [052.11.3 (3R/8)]

Mouse monoclonal [052.11.3 (3R/8)] antibody to Factor I / CFI.

This antibody recognises complement factor I, a member of the peptidase S1 family present in the blood plasma. Factor I is an 88 kDa protein consisting of one polypeptide chain of 35.4 kDa and one of 27.6 kDa. Factor I is a serine protease which inactivates complement components C3b and C4b. C3b cleavage requires the presence of cofactors complement component (3b/4b) receptor 1 (CR1), membrane cofactor protein or factor H, while C4b cleavage requires complement component 4 binding protein or CR1. Factor I has no natural inhibitors.Dysfunctional factor I can lead to uncontrolled activation of the alternative complement pathway causing in systemic depletion of C3 and lower levels of factors B and H, resulting in recurrent pyogenic infections. Defects in CFI are also associated with haemolytic uraemic syndrome. Mouse anti Human Complement Factor I antibody, clone 3R/8 blocks function of complement factor I.Removal of Sodium Azide is recommended prior to use in functional assays.

Flow Cytometry, ELISA, IHC-Fr, WB, Functional Assays

ELISA: 1:500 - 1:4,000

Human

Native human factor I from serum.

Mouse

Monoclonal

052.11.3 (3R/8)

IgG1

Protein A affinity chromatography of ascites.

Lot Specific

Liquid

Supplied in Borate Buffered Saline with 0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human Complement Factor I antibody, clone 052.11.3 (3R/8) recognizes complement factor I, a member of the peptidase S1 family present in the blood plasma. Factor I is an 88 kDa protein consisting of one polypeptide chain of 35.4 kDa and one of 27.6 kDa. Factor I is a serine protease which inactivates complement components C3b and C4b. C3b cleavage requires the presence of cofactors complement component (3b/4b) receptor 1 (CR1), membrane cofactor protein or factor H, while C4b cleavage requires complement component 4 binding protein or CR1. Factor I has no natural inhibitors.Dysfunctional factor I can lead to uncontrolled activation of the alternative complement pathway causing in systemic depletion of C3 and lower levels of factors B and H, resulting in recurrent pyogenic infections. Defects in CFI are also associated with haemolytic uraemic syndrome. Mouse anti Human Complement Factor I antibody, clone 3R/8 blocks function of complement factor I.Removal of Sodium Azide is recommended prior to use in functional assays.