Western blot analysis of extracts of Mouse heart, using Anti-FADD Antibody (A307154) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western blot analysis of extracts of Jurkat cells, using Anti-FADD Antibody (A307154) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence analysis of mouse bone marrow cells using Anti-FADD Antibody (A307154) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 600µg extracts of mouse heart cells using 3µg of Anti-FADD Antibody (A307154). This Western blot was performed on the immunoprecipitate using Anti-FADD Antibody (A307154) at a dilution of 1:1000.
Representative Western blots confirming the absence of SEC62, Fas, and FADD protein expression in Casp3?-Sec62?/?, Casp3?-Fas?/?, and Casp3?-Fadd?/? cells, respectively, compared with wild-type (WT) controls.
Figure 2b from Elise et al. (2026). Originally published in bioRxiv, DOI: https://doi.org/10.64898/2026.06.14.732110. Reproduced under CC BY 4.0.
