Anti-Feline Immunodeficiency Virus gp95 Antibody [SU1-30] (A281779)

Anti-Feline Immunodeficiency Virus gp95 Antibody [SU1-30]

Mouse monoclonal [SU1-30] antibody to Feline Immunodeficiency Virus gp95.

This antibody recognises the relatively conserved E60 peptide (amino acid sequence SSWKQRNRWEWRPDF) located within the V3 region of FIV (Feline Immunodeficiency Virus) envelope glycoprotein SU,gp95. The primary receptor for FIV infection of cats is the T-cell activation antigen CD134 which, along with CXC chemokine receptor 4 (CXCR4), facilitates the infection of CD4+ primary target cells, resulting in a depletion of the CD4+ population. The V3 loop of SU,gp95 is a major immunogenic domain, suggested to contain the main determinants for the binding of FIV SU,gp95 to CXCR4. Mouse anti Feline Immunodeficiency Virus gp95 antibody, clone SU1-30 inhibits the sCD134-activated entry of both clade B and clade C FIV strains (de Parseval, A. et al. 2006). Mouse anti Feline Immunodeficiency Virus gp95 antibody, clone SU1-30 does not recognize Feline Leukaemia Virus, Feline Herpes Virus type 1, Feline Coronavirus or Feline Calicivirus. Removal of sodium azide is recommended prior to use in functional assays.

WB, Functional Assays, Flow Cytometry

Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Viral

Mouse

Monoclonal

SU1-30

IgG1

1 mg/ml

Approximately 95 kDa under reducing conditions

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Feline Immunodeficiency Virus gp95 antibody, clone SU1-30 recognizes the relatively conserved E60 peptide (amino acid sequence SSWKQRNRWEWRPDF) located within the V3 region of FIV (Feline Immunodeficiency Virus) envelope glycoprotein SU,gp95. The primary receptor for FIV infection of cats is the T-cell activation antigen CD134 which, along with CXC chemokine receptor 4 (CXCR4), facilitates the infection of CD4+ primary target cells, resulting in a depletion of the CD4+ population. The V3 loop of SU,gp95 is a major immunogenic domain, suggested to contain the main determinants for the binding of FIV SU,gp95 to CXCR4 (Sundstrom et al. 2008).Mouse anti Feline Immunodeficiency Virus gp95 antibody, clone SU1-30 inhibits the sCD134-activated entry of both clade B and clade C FIV strains (de Parseval, A. et al. 2006).Mouse anti Feline Immunodeficiency Virus gp95 antibody, clone SU1-30 does not recognize Feline Leukaemia Virus, Feline Herpes Virus type 1, Feline Coronavirus or Feline Calicivirus. Removal of sodium azide is recommended prior to use in functional assays.