This antibody reacts with an extracellular epitope of CD334, the fibroblast growth factor receptor 4, which is an approximately 88 kDa receptor tyrosine kinase expressed in variety of tissues.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
NIH 3T3 cells transfected with full length human CD334.
Host
Mouse
Clonality
Monoclonal
Clone ID
4FR6D3
Isotype
IgG1
Light Chains
kappa
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
88 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of CD334 transfected 3T3 cells (red-filled) from nontransfected 3T3 cells (black-dashed) in flow cytometry surface staining of cellular suspension, labeling FGFR4 with Anti-FGFR4 Antibody [4FR6D3] (PE) (A121871), (10 µl reagent per million cells in 100 µl of cell suspension).
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