Anti-FGFR4 Antibody [ARC1474] (A308727)

Name: Anti-FGFR4 Antibody [ARC1474]
Brand: Antibodies.com
SKU: A308727
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC1474] antibody to FGFR4 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-FGFR4 Antibody [ARC1474]

Description

Rabbit monoclonal [ARC1474] antibody to FGFR4.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 700-802 of human FGFR4 (P22455).

Sequence

SLLREGHRMDRPPHCPPELYGLMRECWHAAPSQRPTFKQLVEALDKVLLAVSEEYLDLRLTFGPYSPSGGDASSTCSSSDSVFSHDPLPLGSSSFPFGSGVQT

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1474

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

95 kDa / 125 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CD334, FGFR-4, Fibroblast growth factor receptor 4, JTK2, TKF

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-FGFR4 Antibody [ARC1474] (A308727)

Western blot analysis of extracts of various cell lines, using Anti-FGFR4 Antibody [ARC1474] (A308727) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Western Blot - Anti-FGFR4 Antibody [ARC1474] (A308727)

Western blot analysis of extracts of Mouse lung, using Anti-FGFR4 Antibody [ARC1474] (A308727) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Immunohistochemistry - Anti-FGFR4 Antibody [ARC1474] (A308727)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-FGFR4 Antibody [ARC1474] (A308727) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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