Anti-Fibroblasts/Epithelial Cells Antibody [D7-FIB] (A280222)

Anti-Fibroblasts/Epithelial Cells Antibody [D7-FIB]

Mouse monoclonal [D7-FIB] antibody to Fibroblasts/Epithelial Cells.

The epitope recognized by clone D7-FIB is sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB also binds to epithelium, myoepthelium, smooth muscle and some leucocytes. Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes.

IP, Flow Cytometry, IHC-Fr

Flow Cytometry: 1:50 - 1:200, IHC-Fr 1: 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Human

This antibody does not cross react with Rat or Mouse

Human foreskin fibroblasts.

Mouse

Monoclonal

D7-FIB

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Recognises a ~112kDa molecule expressed on the cell surface of human fibroblasts. The antigen is not expressed by peripheral blood cells, and is found at low levels on a minority of melanoma cell lines.