Anti-FIS1 Antibody (A14996)

Name: Anti-FIS1 Antibody
Brand: Antibodies.com
SKU: A14996
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to FIS1 for WB, ICC/IF, IP and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-FIS1 Antibody

Description

Rabbit polyclonal antibody to FIS1.

Applications

WB, ICC/IF, IP, ELISA

Positive Control

HeLa, 293T, MCF7.

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 0.5µg-4µg antibody for 200µg-400µg extracts of whole cells, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant fusion protein corresponding to amino acids 1-122 of human FIS1.

Sequence

MEAVLNELVSVEDLLKFEKKFQSEKAAGSVSKSTQFEYAWCLVRSKYNDDIRKGIVLLEELLPKGSKEEQRDYVFYLAVGNYRLKEYEKALKYVRGLLQTEPQNNQAKELERLIDKAMKKDG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

17 kDa

Predicted MW

17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

FIS1 homolog, hFis1, Mitochondrial fission 1 protein, Tetratricopeptide repeat protein 11, TPR repeat protein 11, TTC11

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-FIS1 Antibody (A14996)

FIS1 expression in 293T cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-FIS1 Antibody (A14996) at a dilution of 1:1000 and detected with chemiluminescence.

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Immunofluorescence - Anti-FIS1 Antibody (A14996)

FIS1 expression in Hela cells analyzed by immunofluorescence. Staining was performed with Anti-FIS1 Antibody (A14996) at a dilution of 1:50. Nuclei were stained with DAPI (blue).

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Immunoprecipitation - Anti-FIS1 Antibody (A14996)

FIS1 expression in 200 µg extracts of MCF7 cells analyzed by western blot following immunoprecipitation, both using Anti-FIS1 Antibody (A14996) at 3 µg and a dilution of 1:1000, respectively.

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