Anti-FKBP52 Antibody (A14878)

Name: Anti-FKBP52 Antibody
Brand: Antibodies.com
SKU: A14878
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to FKBP52 for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-FKBP52 Antibody

Description

Rabbit polyclonal antibody to FKBP52.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:100, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 220-459 of human FKBP52/FKBP52/FKBP4 (NP_002005.1).

Sequence

YLKPSYAFGSVGKEKFQIPPNAELKYELHLKSFEKAKESWEMNSEEKLEQSTIVKERGTVYFKEGKYKQALLQYKKIVSWLEYESSFSNEEAQKAQALRLASHLNLAMCHLKLQAFSAAIESCNKALELDSNNEKGLFRRGEAHLAVNDFELARADFQKVLQLYPNNKAAKTQLAVCQQRIRRQLAREKKLYANMFERLAEEENKAKAEASSGDHPTDTEMKEEQKSNTAGSQSQVETEA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

52 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

51 kDa FK506-binding protein, 52 kDa FK506-binding protein, 52 kDa FKBP, 59 kDa immunophilin, FK506-binding protein 4, FKBP-4, FKBP-52, FKBP4, FKBP51, FKBP59, p59, Peptidyl-prolyl cis-trans isomerase FKBP4, PPIase FKBP4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-FKBP52 Antibody (A14878)

Western blot analysis of extracts of various cell lines, using Anti-FKBP52 Antibody (A14878) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Immunohistochemistry - Anti-FKBP52 Antibody (A14878)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-FKBP52 Antibody (A14878) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-FKBP52 Antibody (A14878)

Immunofluorescence analysis of U2OS cells using Anti-FKBP52 Antibody (A14878). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-FKBP52 Antibody (A14878)

Immunoprecipitation analysis of 200µg extracts of 293T cells using 1µg of Anti-FKBP52 Antibody (A14878). This Western blot was performed on the immunoprecipitate using Anti-FKBP52 Antibody (A14878) at a dilution of 1:1000.

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