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Anti-FLAG Tag Antibody (A121606)

Western Blot - Anti-FLAG Tag Antibody (A121624) - Antibodies.com Immunofluorescence - Anti-mCherry Antibody - Antibodies.com
$590
Product Datasheet

Goat polyclonal antibody to FLAG Tag for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-FLAG Tag Antibody
Description
Goat polyclonal antibody to FLAG Tag.
Specificity
This antibody recognizes the DYKDDDDK epitope tag fused to either the amino- or carboxy-terminal of recombinant proteins.
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
FLAG tagged proteins.
Immunogen
Recombinant peptide, DYKDDDDK, expressed in and purified from E. coli.
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
3 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 20% Glycerol and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
DDDDK epitope tag, DDDDK tag, DDDK, ddk, DYKDDDDK, DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG
Isotype Controls
Suitable Secondaries
See all Anti-Goat IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-FLAG Tag Antibody (A121624) - Antibodies.comEnlarge Image

Western Blot - Anti-FLAG Tag Antibody (A121606)

293 hTERT RPE-1 cells, transduced with Flag-CAS9 lentivirus, detected with Anti-FLAG Tag Antibody at a 1:1,000 dilution. Lysates at 50µg per lane and rabbit anti-goat IgG antibody (HRP) at a 1:10,000 dilution.
Immunofluorescence - Anti-mCherry Antibody - Antibodies.comEnlarge Image

Immunofluorescence - Anti-FLAG Tag Antibody (A121606)

Fluorescence microscopic images of indicated strains expressing the peroxisomal marker mCherry-PTS (magenta) and the mitochondrial marker mt-GFP (green).
Figure 9 from Bittner et al. (2024). Originally published in PLoS Biol, DOI: 10.1371/journal.pbio.3002508.. Reproduced under CC BY 4.0.
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