Mouse monoclonal [BV10A4] antibody to Flt3/CD135 (APC).
Specificity
This antibody reacts with CD135 (FLT3, FLK2, STK-1), a 130-160 kDa type I transmembrane receptor tyrosine kinase that is involved in early steps of hematopoiesis.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Mouse.
Immunogen
BV-173 leukemic cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
BV10A4
Isotype
IgG1
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
130-160 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of REH cell suspension stained with Anti-CD135 Antibody [BV10A4] (APC) (10µl reagent per milion cells in 100µl of cell suspension).
Separation of REH cells using Anti-CD135 Antibody [BV10A4] (APC) (10µl reagent per milion cells in 100µl of cell suspension, red-filled) from REH cells using Mouse IgG1 [MOPC-21] (APC) (Isotype Control) (concentration in sample is 5µg/ml, same as Anti-CD135 Antibody [BV10A4] (APC) concentration, black-dashed) in flow cytometry analysis of REH cell suspension.
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