Anti-FNIP1 Antibody (A304955)

Immunohistochemistry analysis of human renal cell carcinoma, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-FNIP1 Antibody (A304955) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Immunohistochemistry analysis of human renal cell carcinoma, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-FNIP1 Antibody (A304955) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Western blot analysis of mouse, rat kidney showing detection of ~131 kDa FNIP1 protein using Anti-FNIP1 Antibody (A304955) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse kidney cell lysates. Lane 3: rat kidney cell lysates. Load: 20 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min at room temperature. Predicted/Observed Size: ~131 kDa.

Immunocytochemistry/Immunofluorescence analysis of mouse C2C12 Cells (mouse myoblast cell line), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-FNIP1 Antibody (A304955), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) FNIP1 Antibody (D) Composite.