Anti-FTO Antibody (A8927)

Name: Anti-FTO Antibody
Brand: Antibodies.com
SKU: A8927
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to FTO for WB and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-FTO Antibody

Description

Rabbit polyclonal antibody to FTO.

Applications

WB, IP

Dilutions

WB: 1:500-1:1,000, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human FTO (NP_001073901.1).

Sequence

MKRTPTAEEREREAKKLRLLEELEDTWLPYLTPKDDEFYQQWQLKYPKLILREASSVSEELHKEVQEAFLTLHKHGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSNIKHTEAEIAAACETFLKLNDYLQIETIQALEELAAKEKANEDAVPLCMSADFPRVGMGSSYNGQDEVDIKSRAAYNVTLLNFMDPQKMPYLKEEPYFGMGKMAVSWHHDENLVDRS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

2'-O-methyladenosine-N(6)-)-demethylase FTO, Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein, KIAA1752, m6A(m)-demethylase FTO, mRNA, mRNA N(6)-methyladenosine demethylase FTO, tRNA N1-methyl adenine demethylase FTO, U6 small nuclear RNA, U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-FTO Antibody (A8927)

Western blot analysis of extracts of various cell lines, using Anti-FTO Antibody (A8927) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Western Blot - Anti-FTO Antibody (A8927)

Immunoprecipitation analysis of 300µg extracts of SH-SY5Y cells using 3µg of Anti-FTO Antibody (A8927). This Western blot was performed on the immunoprecipitate using Anti-FTO Antibody (A8927) at a dilution of 1:1000.

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