Rabbit polyclonal antibody to G3BP-1 (Phospho-Ser232)
Specificity
The antibody detects endogenous level of G3BP-1 only when phosphorylated at serine 232.
Applications
WB, IHC, IF
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 232 (S-S-S(p)-P-A) derived from Human G3BP-1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
ATP-dependent DNA helicase VIII, G3BP, GAP SH3 domain-binding protein 1, hDH VIII, Ras GTPase-activating protein-binding protein 1
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP-1 (Phospho-Ser232) Antibody #11082 (left) or the same antibody preincubated with blocking peptide (right).