Rabbit polyclonal antibody to Gab2 (Phospho-Tyr643)
Specificity
The antibody detects endogenous levels of Gab2 only when phosphorylated at tyrosine 643.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 643(V-D-Y(p)-V-Q) derived from Human Gab2 .
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
GRB2-associated binder 2, GRB2-associated-binding protein 2, Growth factor receptor bound protein 2-associated protein 2, KIAA0571, pp100
Western blot analysis of extracts from 3T3 cells (Lane 2), using Gab2 (Phospho-Tyr643) Antibody #11802. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using Gab2 (Phospho-Tyr643) antibody #11802 (left)or the same antibody preincubated with blocking peptide (right).
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