Anti-GAD67 Antibody (A14262)

Name: Anti-GAD67 Antibody
Brand: Antibodies.com
SKU: A14262
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to GAD67 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

GABAergic Neuron Marker Interneuron Marker Purkinje Cells Marker Stellate Cell Marker

Specifications

Name

Anti-GAD67 Antibody

Description

Rabbit polyclonal antibody to GAD67.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human GAD67/GAD67/GAD1 (NP_000808.2).

Sequence

MASSTPSSSATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFKERQSSKNLLSCENSDRDARFRRTETDFSNLFARDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

67 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

67 kDa glutamic acid decarboxylase, GAD, GAD-67, Glutamate decarboxylase 1, Glutamate decarboxylase 67 kDa isoform

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-GAD67 Antibody (A14262)

Western blot analysis of extracts of Mouse lung, using Anti-GAD67 Antibody (A14262) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Western Blot - Anti-GAD67 Antibody (A14262)

Western blot analysis of extracts of 293T cells, using Anti-GAD67 Antibody (A14262) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-GAD67 Antibody (A14262)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-GAD67 Antibody (A14262) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-GAD67 Antibody (A14262)

Immunofluorescence analysis of rat eye cells using Anti-GAD67 Antibody (A14262) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).