Anti-GADD45A Antibody (A13662)

Name: Anti-GADD45A Antibody
Brand: Antibodies.com
SKU: A13662
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to GADD45A for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-GADD45A Antibody

Description

Rabbit polyclonal antibody to GADD45A.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-50 of human GADD45A (NP_001915.1).

Sequence

MTLEEFSAGEQKTERMDKVGDALEEVLSKALSQRTITVGVYEAAKLLNVD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

18 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

DDIT-1, DDIT1, DNA damage-inducible transcript 1 protein, GADD45, Growth arrest and DNA damage-inducible protein GADD45 alpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-GADD45A Antibody (A13662)

Western blot analysis of extracts of various cell lines, using Anti-GADD45A Antibody (A13662) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunohistochemistry - Anti-GADD45A Antibody (A13662)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-GADD45A Antibody (A13662) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-GADD45A Antibody (A13662)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-GADD45A Antibody (A13662) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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