Anti-GAPDS Antibody [Hs-8] (A86462)

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$210

Mouse monoclonal [Hs-8] antibody to GAPDS for WB, ICC and Flow Cytometry.

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Alternative Formats and ConjugatesAlternative Formats

FITC

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-GAPDS Antibody [Hs-8]

Description

Mouse monoclonal [Hs-8] antibody to GAPDS.

Specificity

This antibody reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, previously identified under the general name "intra-acrosomal protein".

Applications

WB, ICC, Flow Cytometry

Dilutions

IHC: 10 µg/ml; membrane permeabilization (acetone) is essential, Flow Cytometry: 3-12 µg/ml. Intraacrosomal staining.

Reactivity

Porcine, Human

Immunogen

Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Host

Mouse

Clonality

Monoclonal

Clone ID

Hs-8

Isotype

IgM

Conjugate

Unconjugated

Purification

Purified by differential precipitation and solid-phase chromatography.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 8, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Store at 2-8°C. Do not freeze!

Synonyms

GAPD2, GAPDH-2, GAPDH2, Glyceraldehyde-3-phosphate dehydrogenase, testis-specific, Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2, Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

Isotype Controls

Mouse IgM [PRF-03] (A86761)

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Flow Cytometry - Anti-GAPDS Antibody [Hs-8] (A86462)

Flow cytometry intracellular staining of human sperm cells staining GAPDS with Anti-GAPDS Antibody [Hs-8] (A86462), (concentration in sample 7.5 µg/ml) GAM APC.

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Flow Cytometry - Anti-GAPDS Antibody [Hs-8] (A86462)

Separation of human GAPDHS positive cells (red-filled) from GAPDHS negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of human sperm cells stained with Anti-GAPDS Antibody [Hs-8] (A86462), (concentration in sample 7.5 µg/ml) GAM APC.

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