Western blot analysis of extracts of various cell lines, using Anti-GATAD2B Antibody (A16332) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded mouse pancreas using Anti-GATAD2B Antibody (A16332) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-GATAD2B Antibody (A16332). This Western blot was performed on the immunoprecipitate using Anti-GATAD2B Antibody (A16332) at a dilution of 1:1000.
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Proteins predicted to interact with GATAD2B
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.