Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
GBP-1, GTP-binding protein 1, Guanine nucleotide-binding protein 1, Guanylate-binding protein 1, hGBP1, HuGBP-1, Interferon-induced guanylate-binding protein 1
Western Blot - Anti-GBP1 Antibody [ARC2521] (A308580)
Western blot analysis of extracts of various cell lines, using Anti-GBP1 Antibody [ARC2521] (A308580) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-GBP1 Antibody [ARC2521] (A308580) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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