Recombinant chimeric monoclonal [6E3] antibody to GC-C.
Applications
WB
Dilutions
WB: 1:1,000
Reactivity
Human
Host
Rabbit / Human
Clonality
Monoclonal
Clone ID
6E3
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
This antibody is a recombinant rabbit chimeric monoclonal antibody containing human IgG1 Fc.
Synonyms
Guanylyl cyclase C, GUC2C, Heat-stable enterotoxin receptor, hSTAR, Intestinal guanylate cyclase, STA receptor, STAR
Western blot analysis of GC-C in HT55, whole cell lysate using Anti-GC-C Antibody [6E3] at 1:1,000 dilution with Goat Anti-Rabbit IgG H&L (HRP) at 1:5,000 dilution. The predicted band weight was 123 kDa, and the observed band weight was 150 kDa.
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