Anti-GCLC Antibody [ARC1065] (A306657)

Name: Anti-GCLC Antibody [ARC1065]
Brand: Antibodies.com
SKU: A306657
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC1065] antibody to GCLC for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-GCLC Antibody [ARC1065]

Description

Rabbit monoclonal [ARC1065] antibody to GCLC.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 538-637 of human GCLC (P48506).

Sequence

LNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEMNYSLILKCNQIANELCECPELLGSAFRKVKYSGSKTDSSN

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1065

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

73 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Gamma-ECS, Gamma-glutamylcysteine synthetase, GCS heavy chain, GLCL, GLCLC, Glutamate--cysteine ligase catalytic subunit

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-GCLC Antibody [ARC1065] (A306657)

Western blot analysis of extracts of Rat kidney, using Anti-GCLC Antibody [ARC1065] (A306657) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Western Blot - Anti-GCLC Antibody [ARC1065] (A306657)

Western blot analysis of extracts of various cell lines, using Anti-GCLC Antibody [ARC1065] (A306657) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Immunohistochemistry - Anti-GCLC Antibody [ARC1065] (A306657)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-GCLC Antibody [ARC1065] (A306657) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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