Anti-Glutaminase Antibody (A11694)

Name: Anti-Glutaminase Antibody
Brand: Antibodies.com
SKU: A11694
Price: 280.0 GBP
Availability: InStock

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$375

Rabbit polyclonal antibody to Glutaminase for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Glutamatergic Neuron Marker

Specifications

Name

Anti-Glutaminase Antibody

Description

Rabbit polyclonal antibody to Glutaminase.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 610-669 of human Glutaminase (Glutaminase (GLS)) (NP_055720.3).

Sequence

KVNPFPKDRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDNGKENQTVHKNLDGLL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

65 - 73 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

GLS, GLS1, Glutaminase kidney isoform, mitochondrial, K-glutaminase, KIAA0838, L-glutamine amidohydrolase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Glutaminase Antibody (A11694)

Western blot analysis of extracts of various cell lines, using Anti-Glutaminase Antibody (A11694) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunofluorescence - Anti-Glutaminase Antibody (A11694)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Glutaminase Antibody (A11694) at a dilution of 1:150. DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Glutaminase Antibody (A11694)

Immunofluorescence analysis of PC-12 cells using Anti-Glutaminase Antibody (A11694) at a dilution of 1:150. DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Glutaminase Antibody (A11694)

Immunofluorescence analysis of SH-SY5Y cells using Anti-Glutaminase Antibody (A11694) at a dilution of 1:150. DAPI was used to stain the cell nuclei (blue).

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