Anti-GM130 Antibody [ARC0589] (A308003)

Name: Anti-GM130 Antibody [ARC0589]
Brand: Antibodies.com
SKU: A308003
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC0589] antibody to GM130 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Golgi Marker

Specifications

Name

Anti-GM130 Antibody [ARC0589]

Description

Rabbit monoclonal [ARC0589] antibody to GM130.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GM130 (Q08379).

Sequence

MWPQPRLPPRPAMSEETRQSKLAAAKKKLREYQQRNSPGVPTGAKKKKKIKNGSNPETTTSGGCHSPEDTPKDNAATLQPSDDTVLPGGVPSPGASLTSM

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0589

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

130 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

130 kDa cis-Golgi matrix protein, GM130 autoantigen, GOLGA2, Golgin subfamily A member 2, Golgin-95

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-GM130 Antibody [ARC0589] (A308003)

Western blot analysis of extracts of various cell lines, using Anti-GM130 Antibody [ARC0589] (A308003) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Western Blot - Anti-GM130 Antibody [ARC0589] (A308003)

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Immunohistochemistry - Anti-GM130 Antibody [ARC0589] (A308003)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-GM130 Antibody [ARC0589] (A308003) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-GM130 Antibody [ARC0589] (A308003)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-GM130 Antibody [ARC0589] (A308003) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.