Anti-GNAI3 Antibody (A13745)

Name: Anti-GNAI3 Antibody
Brand: Antibodies.com
SKU: A13745

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This product is discontinued

Anti-GNAI3 Antibody (A13745) has been discontinued and is no longer available.

View all GNAI3 Antibodies.

Specifications

Name

Anti-GNAI3 Antibody

Description

Rabbit polyclonal antibody to GNAI3.

Applications

WB, IHC

Dilutions

WB: 1:1,000-1:2,000, IHC: 1:50-1:100

Reactivity

Human, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GNAI3 (NP_006487.1).

Sequence

MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAAR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

41 - 45 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

G(i) alpha-3, Guanine nucleotide-binding protein G(i) subunit alpha-3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-GNAI3 Antibody (A13745)

Western blot analysis of extracts of various cell lines, using Anti-GNAI3 Antibody (A13745) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.

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Immunohistochemistry - Anti-GNAI3 Antibody (A13745)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-GNAI3 Antibody (A13745) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-GNAI3 Antibody (A13745)

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-GNAI3 Antibody (A13745) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.