Anti-Golgin-245 Antibody (A11266)

Name: Anti-Golgin-245 Antibody
Brand: Antibodies.com
SKU: A11266
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to Golgin-245 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Golgin-245 Antibody

Description

Rabbit polyclonal antibody to Golgin-245.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GOLGA4 (NP_002069.2).

Sequence

MFKKLKQKISEEQQQLQQALAPAQASSNSSTPTRMRSRTSSFTEQLDEGTPNRESGDTQSFAQKLQLRVPSVESLFRSPIKESLFRSSSKESLVRTSSRESLNRLDLDSSTASFDPPSDMDSEAEDLVGNSDSLNKEQLIQRLRRMERSL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

280 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

256 kDa golgin, Golgin subfamily A member 4, Protein 72.1, Trans-Golgi p230

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Golgin-245 Antibody (A11266)

Western blot analysis of extracts of various cell lines, using Anti-Golgin-245 Antibody (A11266) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Immunofluorescence - Anti-Golgin-245 Antibody (A11266)

Immunofluorescence analysis of C6 cells using Anti-Golgin-245 Antibody (A11266) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Golgin-245 Antibody (A11266)

Immunofluorescence analysis of HeLa cells using Anti-Golgin-245 Antibody (A11266) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Golgin-245 Antibody (A11266)

Immunofluorescence analysis of L929 cells using Anti-Golgin-245 Antibody (A11266) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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