This antibody binds specifically to the C-terminal domain of human GPCR GPR12, detecting the non-phosphorylated form. It supports Western blot analysis, enables immunoprecipitation from tissue lysates, and is applicable for immunohistochemistry in cultured cells and tissue sections.
Applications
WB, IHC
Dilutions
WB: 1,000, IHC: 1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human GPCR GPR12. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot validation of GPCR GPR12 Receptor (GPCR GPR12) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing GPCR GPR12 were probed with the phosphorylation-independent anti-GPCR GPR12 antibody (A334456) at a 1:1000 dilution.
Immunohistochemical detection of GPCR GPR12 Receptor (GPCR GPR12) in kidney. Kidney sections were dewaxed, microwaved in citric acid, and incubated with anti-GPCR GPR12 antibody (A334456) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin.
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