This antibody specifically recognizes the C-terminal region of human GPCR GPR15, detecting the non-phosphorylated form. It is suitable for Western blot analysis, supports immunoprecipitation from tissue lysates, and is optimized for immunohistochemistry in cultured cells and tissue sections.
Applications
WB, IHC
Dilutions
WB: 1,000, IHC: 1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human GPCR GPR15. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
BoB, Brother of Bonzo, G-protein coupled receptor 15, GPR15
Western blot analysis of GPCR GPR15 Receptor (GPCR GPR15) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing GPCR GPR15 were immunoblotted with the phosphorylation-independent anti-GPCR GPR15 antibody (A334458) at a 1:1000 dilution.
Immunohistochemical localization of GPCR GPR15 Receptor (GPCR GPR15) in pancreas. Pancreatic sections were dewaxed, microwaved in citric acid, and stained with anti-GPCR GPR15 antibody (A334458) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin.
Detection of GPCR GPR15 Receptor (GPCR GPR15) in kidney by immunohistochemistry. Kidney sections were dewaxed, microwaved in citric acid, and incubated with anti-GPCR GPR15 antibody (A334458) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin.
Publishing research using Anti-GPCR GPR15 Antibody (A334458)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-GPCR GPR15 Antibody (A334458)
