GPCR GPR17 expression in Mouse Brain lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-GPCR GPR17 Antibody (A84726) at 3µg/ml and detected by chemiluminescence.
GPCR GPR17 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-GPCR GPR17 Antibody (A84726) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
GPCR GPR17 expression in U251 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-GPCR GPR17 Antibody (A84726) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Plasma membrane and vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
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