This antibody binds specifically to the C-terminal domain of human GPCR GPR25, detecting the non-phosphorylated isoform. It is optimized for Western blot analysis, enables immunoprecipitation from tissue lysates, and is suitable for immunohistochemistry in cultured cells and tissue sections.
Applications
WB, IHC
Dilutions
WB: 1,000, IHC: 1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human GPCR GPR25. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot validation of GPCR GPR25 Receptor (GPCR GPR25) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing GPCR GPR25 were probed with the phosphorylation-independent anti-GPCR GPR25 antibody (A334527) at a 1:1000 dilution.
Immunohistochemical detection of GPCR GPR25 Receptor (GPCR GPR25) in small intestine. Small intestine sections were dewaxed, microwaved in citric acid, and incubated with anti-GPCR GPR25 antibody (A334527) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin.
Publishing research using Anti-GPCR GPR25 Antibody (A334527)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-GPCR GPR25 Antibody (A334527)
