Anti-GPCR GPR25 Antibody (A334527)

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This product is discontinued

Anti-GPCR GPR25 Antibody (A334527) has been discontinued and is no longer available.

View all GPCR GPR25 Antibodies.

Specifications

Name

Anti-GPCR GPR25 Antibody

Description

Rabbit polyclonal antibody to GPCR GPR25.

Specificity

This antibody binds specifically to the C-terminal domain of human GPCR GPR25, detecting the non-phosphorylated isoform. It is optimized for Western blot analysis, enables immunoprecipitation from tissue lysates, and is suitable for immunohistochemistry in cultured cells and tissue sections.

Applications

WB, IHC

Dilutions

WB: 1,000, IHC: 1:100

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the C-terminal of human GPCR GPR25. Immunogen range is 22-22 amino acids.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Antigen affinity purification.

Concentration

Lot Specific

Product Form

Liquid

Formulation

Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

GPR25, Probable G-protein coupled receptor 25

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Anti-GPCR GPR25 Antibody (A334527)

Western blot validation of GPCR GPR25 Receptor (GPCR GPR25) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing GPCR GPR25 were probed with the phosphorylation-independent anti-GPCR GPR25 antibody (A334527) at a 1:1000 dilution.

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Immunohistochemistry - Anti-GPCR GPR25 Antibody (A334527)

Immunohistochemical detection of GPCR GPR25 Receptor (GPCR GPR25) in small intestine. Small intestine sections were dewaxed, microwaved in citric acid, and incubated with anti-GPCR GPR25 antibody (A334527) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin.