This antibody targets the C-terminal domain of human GPCR GPR27, recognizing the non-phosphorylated isoform. It is suitable for Western blot analysis, facilitates immunoprecipitation from tissue lysates, and is optimized for immunohistochemistry in cultured cells and tissue sections.
Applications
WB, IHC
Dilutions
WB: 1,000, IHC: 1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human GPCR GPR27. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
GPR27, Probable G-protein coupled receptor 27, SREB1, Super conserved receptor expressed in brain 1
Western blot analysis of GPCR GPR27 Receptor (GPCR GPR27) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing GPCR GPR27 were immunoblotted with the phosphorylation-independent anti-GPCR GPR27 antibody (A334528) at a 1:1000 dilution.
Immunohistochemical localization of GPCR GPR27 Receptor (GPCR GPR27) in small intestine. Small intestine sections were dewaxed, microwaved in citric acid, and stained with anti-GPCR GPR27 antibody (A334528) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin.
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