This antibody targets the C-terminal region of human GPCR GPR32, recognizing the non-phosphorylated form. It is suitable for Western blot analysis, supports immunoprecipitation from tissue lysates, and is optimized for immunohistochemistry in cultured cells and tissue sections.
Applications
WB, IHC
Dilutions
WB: 1,000, IHC: 1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human GPCR GPR32. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot confirmation of G protein-coupled receptor 32 (GPCR GPR32) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing GPCR GPR32 were probed with the phosphorylation-independent anti-GPCR GPR32 antibody (A334463) at a 1:1000 dilution.
Immunohistochemical detection of GPCR GPR32 Receptor (GPCR GPR32) in pancreas. Pancreatic sections were dewaxed, microwaved in citric acid, and incubated with anti-GPCR GPR32 antibody (A334463) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin.
Localization of GPCR GPR32 Receptor (GPCR GPR32) in small intestine by immunohistochemistry. Small intestine sections were dewaxed, microwaved in citric acid, and stained with anti-GPCR GPR32 antibody (A334463) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin.
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