This antibody specifically recognizes the C-terminal region of human GPCR GPR6, detecting the non-phosphorylated form. It is validated for Western blot analysis, supports immunoprecipitation from tissue lysates, and is suitable for immunohistochemistry in cultured cells and tissue sections.
Applications
WB, IHC
Dilutions
WB: 1,000, IHC: 1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human GPCR GPR6. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot confirmation of GPCR GPR6 Receptor (GPCR GPR6) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing GPCR GPR6 were immunoblotted with the phosphorylation-independent anti-GPCR GPR6 antibody (A334468) at a 1:1000 dilution.
Immunohistochemical localization of GPCR GPR6 Receptor (GPCR GPR6) in small intestine. Small intestine sections were dewaxed, microwaved in citric acid, and stained with anti-GPCR GPR6 antibody (A334468) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin.
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