This antibody specifically recognizes the C-terminal domain of human GPR158, detecting the non-phosphorylated form. It is validated for Western blot analysis and supports immunoprecipitation from tissue lysates.
Applications
WB
Dilutions
WB: 1:1,000
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human GPR158. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot validation of GPR158/mGlyR Receptor (GPR158) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing GPR158/mGlyR were probed with the phosphorylation-independent anti-GPR158/mGlyR antibody (A334524) at a 1:1000 dilution.
Immunohistochemical localization of GPR158/mGlyR Receptor (GPR158/mGlyR) in kidney. Kidney sections were dewaxed, microwaved in citric acid, and stained with anti-GPR158/mGlyR antibody (A334524) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin.
Detection of GPR158/mGlyR Receptor (GPR158) in small intestine by immunohistochemistry. Small intestine sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR158/mGlyR antibody (A334524) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin.
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