This antibody targets the C-terminal region of human GPR22, recognizing the non-phosphorylated form. It is validated for Western blot analysis, supports immunoprecipitation from tissue lysates, and is suitable for immunohistochemistry in cultured cells and tissue sections.
Applications
WB, IHC
Dilutions
WB: 1,000, IHC: 1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human GPR22. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot confirmation of GPR22 Receptor (GPR22) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing GPR22 were immunoblotted with the phosphorylation-independent anti-GPR22 antibody (A334460) at a 1:1000 dilution.
Immunohistochemical localization of GPR22 Receptor (GPR22) in pancreas. Pancreatic sections were dewaxed, microwaved in citric acid, and stained with anti-GPR22 antibody (A334460) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin.
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Proteins predicted to interact with GPR22
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
