Anti-GPR35 Antibody (A334529)

Detection of non-phosphorylated human GPR35 Receptor (GPR35) by Western blot. Flp-In TREx 293 cells harboring human GPR35-eYFP, induced to express the receptor, were treated with vehicle or 100 nM Lodoxamide for 5 min. Lysates were enriched with a GFP-trap and immunoblotted with anti-GPR35 antibody (A334529) at 1:1000 (upper panel) or anti-GFP at 1:10,000 to confirm expression and equal loading (lower panel).

Western blot analysis of non-phosphorylated mouse GPR35 Receptor (GPR35). Flp-In TREx 293 cells harboring mouse GPR35-HA, induced to express the receptor, were treated with vehicle or 10 µM Zaprinast for 5 min. Lysates were enriched with an HA-trap and probed with anti-GPR35 antibody (A334529) at 1:1000 (upper panel) or anti-HA at 1:10,000 to confirm expression and equal loading (lower panel).

Immunohistochemical localization of GPR35 Receptor (GPR35) in human colon. Colon sections were dewaxed, microwaved in citric acid, and stained with anti-GPR35 antibody (A334529) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed with 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. GPR35 receptors were detected at the plasma membrane of epithelial cells and macrophages.

Detection of GPR35 Receptor (GPR35) in Crohn’s disease by immunohistochemistry. Colon sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR35 antibody (A334529) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed with 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. GPR35 receptors were observed at the plasma membrane of epithelial cells and macrophages.